5FIQ

Exonuclease domain-containing 1 (Exd1) in the native conformation

Summary for 5FIQ

• Entry DOI: 10.2210/pdb5fiq/pdb
• Related: 5FIS
• Descriptor: EXD1 (2 entities in total)
• Functional Keywords: hydrolase, exonuclease, pirna biogenesis, dimer, rna binding
• Biological source: BOMBYX MORI (DOMESTIC SILKWORM)
• Total number of polymer chains: 5
• Total formula weight: 140512.23

Authors

Yang, Z.,Chen, K.M.,Pandey, R.R.,Homolka, D.,Reuter, M.,Rodino Janeiro, B.K.,Sachidanandam, R.,Fauvarque, M.O.,McCarthy, A.A.,Pillai, R.S. (deposition date: 2015-10-01, release date: 2015-12-23, Last modification date: 2024-01-10)

Primary citation

Yang, Z.,Chen, K.M.,Pandey, R.R.,Homolka, D.,Reuter, M.,Rodino Janeiro, B.K.,Sachidanandam, R.,Fauvarque, M.O.,Mccarthy, A.A.,Pillai, R.S.
Piwi Slicing and Exd1 Drive Biogenesis of Nuclear Pirnas from Cytosolic Targets of the Mouse Pirna Pathway
Mol.Cell, 61:138-, 2016

PubMed Abstract: PIWI-interacting RNAs (piRNAs) guide PIWI proteins to suppress transposons in the cytoplasm and nucleus of animal germ cells, but how silencing in the two compartments is coordinated is not known. Here we demonstrate that endonucleolytic slicing of a transcript by the cytosolic mouse PIWI protein MILI acts as a trigger to initiate its further 5'→3' processing into non-overlapping fragments. These fragments accumulate as new piRNAs within both cytosolic MILI and the nuclear MIWI2. We also identify Exonuclease domain-containing 1 (EXD1) as a partner of the MIWI2 piRNA biogenesis factor TDRD12. EXD1 homodimers are inactive as a nuclease but function as an RNA adaptor within a PET (PIWI-EXD1-Tdrd12) complex. Loss of Exd1 reduces sequences generated by MILI slicing, impacts biogenesis of MIWI2 piRNAs, and de-represses LINE1 retrotransposons. Thus, piRNA biogenesis triggered by PIWI slicing, and promoted by EXD1, ensures that the same guides instruct PIWI proteins in the nucleus and cytoplasm.

PubMed: 26669262
DOI: 10.1016/J.MOLCEL.2015.11.009

Experimental method

X-RAY DIFFRACTION (2.4 Å)