5FG3

Crystal structure of GDP-bound aIF5B from Aeropyrum pernix

5FG3 の概要

• エントリーDOI: 10.2210/pdb5fg3/pdb
• 分子名称: Probable translation initiation factor IF-2, GUANOSINE-5'-DIPHOSPHATE (3 entities in total)
• 機能のキーワード: translation initiaton factor, gtpase, ribosomal subunit joining, crenarchaea, translation
• 由来する生物種: Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 69898.92

構造登録者

Murakami, R.,Miyoshi, T.,Uchiumi, T.,Ito, K. (登録日: 2015-12-20, 公開日: 2016-05-04, 最終更新日: 2023-11-08)

主引用文献

Murakami, R.,Miyoshi, T.,Uchiumi, T.,Ito, K.
Crystal structure of translation initiation factor 5B from the crenarchaeon Aeropyrum pernix.
Proteins, 84:712-717, 2016

PubMed Abstract: Initiation factor 5B (IF5B) is a universally conserved translational GTPase that catalyzes ribosomal subunit joining. In eukaryotes, IF5B directly interacts via a groove in its domain IV with initiation factor 1A (IF1A), another universally conserved initiation factor, to accomplish efficient subunit joining. Here, we have determined the first structure of a crenarchaeal IF5B, which revealed that the archaea-specific region of IF5B (helix α15) binds and occludes the groove of domain IV. Therefore, archaeal IF5B cannot access IF1A in the same manner as eukaryotic IF5B. This fact suggests that different relationships between IF5B and IF1A exist in archaea and eukaryotes.

PubMed: 26868175
DOI: 10.1002/prot.25009

実験手法

X-RAY DIFFRACTION (1.9 Å)