5FFJ

Structure of a nuclease-deletion mutant of the Type ISP restriction-modification enzyme LlaGI in complex with a DNA substrate mimic

Summary for 5FFJ

• Entry DOI: 10.2210/pdb5ffj/pdb
• Related: 4XQK
• Descriptor: Endonuclease and methylase LlaGI, DNA (5'-D(P*TP*CP*CP*TP*CP*CP*AP*TP*CP*CP*AP*GP*TP*CP*TP*AP*TP*TP*AP*GP*CP*T)-3'), DNA (5'-D(P*TP*AP*GP*CP*TP*AP*AP*TP*AP*GP*AP*CP*TP*GP*GP*AP*TP*GP*GP*AP*GP*G)-3'), ... (4 entities in total)
• Functional Keywords: helicase-like atpase, methyltransferase, dna-binding protein, restriction-modification enzyme, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Lactococcus lactis; More
• Total number of polymer chains: 6
• Total formula weight: 349661.66

Authors

Saikrishnan, K.,Kulkarni, M.,Nirwan, N. (deposition date: 2015-12-18, release date: 2016-03-30, Last modification date: 2024-03-20)

• Primary citation: Kulkarni, M.,Nirwan, N.,van Aelst, K.,Szczelkun, M.D.,Saikrishnan, K.; Structural insights into DNA sequence recognition by Type ISP restriction-modification enzymes; Nucleic Acids Res., 44:4396-4408, 2016; PubMed: 26975655; DOI: 10.1093/nar/gkw154

Experimental method

X-RAY DIFFRACTION (2.84 Å)