5FEB

Crystal structure of the Voltage-gated Sodium Channel Beta 2 subunit extracellular domain

5FEB の概要

• エントリーDOI: 10.2210/pdb5feb/pdb
• 関連するPDBエントリー: 5FDY
• 分子名称: Sodium channel subunit beta-2, GLYCEROL (3 entities in total)
• 機能のキーワード: membrane protein ion channel sodium, membrane protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: O60939
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14653.57

構造登録者

Das, S.,Van Petegem, F. (登録日: 2015-12-16, 公開日: 2016-03-09, 最終更新日: 2024-10-09)

主引用文献

Das, S.,Gilchrist, J.,Bosmans, F.,Van Petegem, F.
Binary architecture of the Nav1.2-beta 2 signaling complex.
Elife, 5:-, 2016

PubMed Abstract: To investigate the mechanisms by which β-subunits influence Nav channel function, we solved the crystal structure of the β2 extracellular domain at 1.35Å. We combined these data with known bacterial Nav channel structural insights and novel functional studies to determine the interactions of specific residues in β2 with Nav1.2. We identified a flexible loop formed by (72)Cys and (75)Cys, a unique feature among the four β-subunit isoforms. Moreover, we found that (55)Cys helps to determine the influence of β2 on Nav1.2 toxin susceptibility. Further mutagenesis combined with the use of spider toxins reveals that (55)Cys forms a disulfide bond with (910)Cys in the Nav1.2 domain II pore loop, thereby suggesting a 1:1 stoichiometry. Our results also provide clues as to which disulfide bonds are formed between adjacent Nav1.2 (912/918)Cys residues. The concepts emerging from this work will help to form a model reflecting the β-subunit location in a Nav channel complex.

PubMed: 26894959
DOI: 10.7554/eLife.10960

実験手法

X-RAY DIFFRACTION (1.35 Å)