5F9X

X-RAY STRUCTURE OF THE ADDUCT BETWEEN HEN EGG WHITE LYSOZYME AND CISPLATIN UPON 24 HOURS OF INCUBATION AT 55 DEGREES

Summary for 5F9X

• Entry DOI: 10.2210/pdb5f9x/pdb
• Related: 5F9U
• Descriptor: Lysozyme C, GLYCEROL, Cisplatin, ... (4 entities in total)
• Functional Keywords: hydrolase, drug, cisplatin
• Biological source: Gallus gallus (Chicken)
• Total number of polymer chains: 1
• Total formula weight: 15023.34

Authors

Russo Krauss, I.,Ferraro, G.,Pica, A.,Merlino, A. (deposition date: 2015-12-10, release date: 2016-04-13, Last modification date: 2024-11-20)

Primary citation

Ferraro, G.,Pica, A.,Russo Krauss, I.,Pane, F.,Amoresano, A.,Merlino, A.
Effect of temperature on the interaction of cisplatin with the model protein hen egg white lysozyme.
J.Biol.Inorg.Chem., 21:433-442, 2016

PubMed Abstract: The products of the reaction between cisplatin (CDDP) and the model protein hen egg white lysozyme (HEWL) at 20, 37 and 55 °C in pure water were studied by UV-Vis absorption spectroscopy, intrinsic fluorescence and circular dichroism, dynamic and electrophoretic light scattering and inductively coupled plasma mass spectrometry. X-ray structures were also solved for the adducts formed at 20 and 55 °C. Data demonstrate that high temperature facilitates the formation of CDDP-HEWL adducts, where Pt atoms bind ND1 atom of His15 or NE2 atom of His15 and NH1 atom of Arg14. Our study suggests that high human body temperature (fever) could increase the rate of drug binding to proteins thus enhancing possible toxic side effects related to CDDP administration.

PubMed: 27040953
DOI: 10.1007/s00775-016-1352-0

Experimental method

X-RAY DIFFRACTION (1.94 Å)