5F99

X-ray Structure of the MMTV-A Nucleosome Core Particle

Summary for 5F99

• Entry DOI: 10.2210/pdb5f99/pdb
• Descriptor: Histone H3.2, Histone H4, Histone H2A type 1, ... (9 entities in total)
• Functional Keywords: nucleosome core particle histone dna, dna binding protein
• Biological source: Xenopus laevis (African clawed frog); More
• Cellular location: Nucleus: P84233 P62799 P06897 P02281
• Total number of polymer chains: 10
• Total formula weight: 199293.58

Authors

Frouws, T.D.,Richmond, T.J. (deposition date: 2015-12-09, release date: 2016-02-03, Last modification date: 2024-01-10)

Primary citation

Frouws, T.D.,Duda, S.C.,Richmond, T.J.
X-ray structure of the MMTV-A nucleosome core.
Proc.Natl.Acad.Sci.USA, 113:1214-1219, 2016

PubMed Abstract: The conformation of DNA bound in nucleosomes depends on the DNA sequence. Questions such as how nucleosomes are positioned and how they potentially bind sequence-dependent nuclear factors require near-atomic resolution structures of the nucleosome core containing different DNA sequences; despite this, only the DNA for two similar α-satellite sequences and a sequence (601) selected in vitro have been visualized bound in the nucleosome core. Here we report the 2.6-Å resolution X-ray structure of a nucleosome core particle containing the DNA sequence of nucleosome A of the 3'-LTR of the mouse mammary tumor virus (147 bp MMTV-A). To our knowledge, this is the first nucleosome core particle structure containing a promoter sequence and crystallized from Mg(2+) ions. It reveals sequence-dependent DNA conformations not seen previously, including kinking into the DNA major groove.

PubMed: 26787910
DOI: 10.1073/pnas.1524607113

Experimental method

X-RAY DIFFRACTION (2.63 Å)