5F6C

The structure of E. coli RNase E catalytically inactive mutant with RNA bound

Summary for 5F6C

DescriptorRibonuclease E, RNA (5'-R(P*GP*U)-3'), RNA (5'-R(P*GP*UP*G)-3'), ... (7 entities in total)
Functional Keywordsribonuclease, hydrolytic mechanism, regulatory rna, hydrolase
Biological sourceEscherichia coli (strain K12)
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Total number of polymer chains4
Total molecular weight117324.74
Authors
Bandyra, K.J.,Luisi, B.F. (deposition date: 2015-12-05, release date: 2016-12-14, Last modification date: 2019-02-27)
Primary citation
Bandyra, K.J.,Wandzik, J.M.,Luisi, B.F.
Substrate Recognition and Autoinhibition in the Central Ribonuclease RNase E.
Mol. Cell, 72:275-285.e4, 2018
PubMed: 30270108 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2018.08.039
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.002 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.24910 1.8% 3.6% 0.7% 0.30MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution