5F09

Structure of inactive GCPII mutant in complex with beta-citryl glutamate

5F09 の概要

• エントリーDOI: 10.2210/pdb5f09/pdb
• 分子名称: Glutamate carboxypeptidase 2, TRIETHYLENE GLYCOL, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: substrate, inactive variant, metallopeptidase, hydrolase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cell membrane ; Single-pass type II membrane protein . Isoform PSMA': Cytoplasm : Q04609
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 86667.93

構造登録者

Tykvart, J.,Navratil, M.,Pachl, P.,Konvalinka, J. (登録日: 2015-11-27, 公開日: 2016-06-01, 最終更新日: 2024-10-16)

主引用文献

Navratil, M.,Tykvart, J.,Schimer, J.,Pachl, P.,Navratil, V.,Rokob, T.A.,Hlouchova, K.,Rulisek, L.,Konvalinka, J.
Comparison of human glutamate carboxypeptidases II and III reveals their divergent substrate specificities.
Febs J., 283:2528-2545, 2016

PubMed Abstract: Glutamate carboxypeptidase III (GCPIII) is best known as a homologue of glutamate carboxypeptidase II [GCPII; also known as prostate-specific membrane antigen (PSMA)], a protease involved in neurological disorders and overexpressed in a number of solid cancers. However, mouse GCPIII was recently shown to cleave β-citrylglutamate (BCG), suggesting that these two closely related enzymes have distinct functions. To develop a tool to dissect, evaluate and quantify the activities of human GCPII and GCPIII, we analysed the catalytic efficiencies of these enzymes towards three physiological substrates. We observed a high efficiency of BCG cleavage by GCPIII but not GCPII. We also identified a strong modulation of GCPIII enzymatic activity by divalent cations, while we did not observe this effect for GCPII. Additionally, we used X-ray crystallography and computational modelling (quantum and molecular mechanical calculations) to describe the mechanism of BCG binding to the active sites of GCPII and GCPIII, respectively. Finally, we took advantage of the substantial differences in the enzymatic efficiencies of GCPII and GCPIII towards their substrates, using enzymatic assays for specific detection of these proteins in human tissues. Our findings suggest that GCPIII may not act merely as a complementary enzyme to GCPII, and it more likely possesses a specific physiological function related to BCG metabolism in the human body.

PubMed: 27208881
DOI: 10.1111/febs.13761

実験手法

X-RAY DIFFRACTION (1.85 Å)