5EYB

X-ray Structure of Reb1-Ter Complex

5EYB の概要

• エントリーDOI: 10.2210/pdb5eyb/pdb
• 分子名称: DNA-binding protein reb1, DNA (26-MER), ... (4 entities in total)
• 機能のキーワード: protein-dna complex, transcription termination, replication termination, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 118044.90

構造登録者

Jaiswal, R.,Choudhury, M.,Zaman, S.,Singh, S.,Santosh, V.,Bastia, D.,Escalante, C.R. (登録日: 2015-11-24, 公開日: 2016-04-13, 最終更新日: 2024-03-06)

主引用文献

Jaiswal, R.,Choudhury, M.,Zaman, S.,Singh, S.,Santosh, V.,Bastia, D.,Escalante, C.R.
Functional architecture of the Reb1-Ter complex of Schizosaccharomyces pombe.
Proc.Natl.Acad.Sci.USA, 113:E2267-E2276, 2016

PubMed Abstract: Reb1 ofSchizosaccharomyces pomberepresents a family of multifunctional proteins that bind to specific terminator sites (Ter) and cause polar termination of transcription catalyzed by RNA polymerase I (pol I) and arrest of replication forks approaching the Ter sites from the opposite direction. However, it remains to be investigated whether the same mechanism causes arrest of both DNA transactions. Here, we present the structure of Reb1 as a complex with a Ter site at a resolution of 2.7 Å. Structure-guided molecular genetic analyses revealed that it has distinct and well-defined DNA binding and transcription termination (TTD) domains. The region of the protein involved in replication termination is distinct from the TTD. Mechanistically, the data support the conclusion that transcription termination is not caused by just high affinity Reb1-Ter protein-DNA interactions. Rather, protein-protein interactions between the TTD with the Rpa12 subunit of RNA pol I seem to be an integral part of the mechanism. This conclusion is further supported by the observation that double mutations in TTD that abolished its interaction with Rpa12 also greatly reduced transcription termination thereby revealing a conduit for functional communications between RNA pol I and the terminator protein.

PubMed: 27035982
DOI: 10.1073/pnas.1525465113

実験手法

X-RAY DIFFRACTION (2.7 Å)