5EYB
X-ray Structure of Reb1-Ter Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-03-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9762, 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.959, 162.911, 71.086 |
| Unit cell angles | 90.00, 94.66, 90.00 |
Refinement procedure
| Resolution | 32.490 - 2.700 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.572 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.060 | 0.645 |
| Number of reflections | 42323 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 298 | AMMONIUM SULFATE, PEG 5000 MME, MES, MICROBATCH, TEMPERATURE 293K |
