5EWR
C merolae U4 snRNP protein Snu13
Summary for 5EWR
| Entry DOI | 10.2210/pdb5ewr/pdb |
| Descriptor | Box C/D snoRNP and U4 snRNP component Snu13p (2 entities in total) |
| Functional Keywords | splicing, rna binding protein |
| Biological source | Cyanidioschyzon merolae (Red alga) |
| Total number of polymer chains | 1 |
| Total formula weight | 15790.30 |
| Authors | Garside, E.L.,Black, C.,Rader, S.,MacMillan, A.M. (deposition date: 2015-11-20, release date: 2016-04-20, Last modification date: 2023-09-27) |
| Primary citation | Black, C.S.,Garside, E.L.,MacMillan, A.M.,Rader, S.D. Conserved structure of Snu13 from the highly reduced spliceosome of Cyanidioschyzon merolae. Protein Sci., 25:911-916, 2016 Cited by PubMed Abstract: Structural and functional analysis of proteins involved in pre-mRNA splicing is challenging because of the complexity of the splicing machinery, known as the spliceosome. Bioinformatic, proteomic, and biochemical analyses have identified a minimal spliceosome in the red alga Cyanidioschyzon merolae. This spliceosome consists of only 40 core proteins, compared to ∼ 70 in S. cerevisiae (yeast) and ∼ 150 in humans. We report the X-ray crystallographic analysis of C. merolae Snu13 (CmSnu13), a key component of the assembling spliceosome, and present evidence for conservation of Snu13 function in this algal splicing pathway. The near identity of CmSnu13's three-dimensional structure to yeast and human Snu13 suggests that C. merolae should be an excellent model system for investigating the structure and function of the conserved core of the spliceosome. PubMed: 26833716DOI: 10.1002/pro.2894 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.35 Å) |
Structure validation
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