5EW2

Human thrombin sandwiched between two DNA aptamers: HD22 and HD1-deltaT12

5EW2 の概要

• エントリーDOI: 10.2210/pdb5ew2/pdb
• 関連するBIRD辞書のPRD_ID: PRD_000020
• 分子名称: thrombin light chain, Thrombin heavy chain, HD22 (27mer), ... (8 entities in total)
• 機能のキーワード: protein-dna complex, blood coagulation, dna aptamer, dna-inhibitor, g-quadruplex, duplex-quadruplex junction, serine protease, hydrolase, abasic furan, hydrolase-dna complex, protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47725.30

構造登録者

Pica, A.,Russo Krauss, I.,Parente, V.,Sica, F. (登録日: 2015-11-20, 公開日: 2016-11-30, 最終更新日: 2024-10-16)

主引用文献

Pica, A.,Russo Krauss, I.,Parente, V.,Tateishi-Karimata, H.,Nagatoishi, S.,Tsumoto, K.,Sugimoto, N.,Sica, F.
Through-bond effects in the ternary complexes of thrombin sandwiched by two DNA aptamers.
Nucleic Acids Res., 45:461-469, 2017

PubMed Abstract: Aptamers directed against human thrombin can selectively bind to two different exosites on the protein surface. The simultaneous use of two DNA aptamers, HD1 and HD22, directed to exosite I and exosite II respectively, is a very powerful approach to exploit their combined affinity. Indeed, strategies to link HD1 and HD22 together have been proposed in order to create a single bivalent molecule with an enhanced ability to control thrombin activity. In this work, the crystal structures of two ternary complexes, in which thrombin is sandwiched between two DNA aptamers, are presented and discussed. The structures shed light on the cross talk between the two exosites. The through-bond effects are particularly evident at exosite II, with net consequences on the HD22 structure. Moreover, thermodynamic data on the binding of the two aptamers are also reported and analyzed.

PubMed: 27899589
DOI: 10.1093/nar/gkw1113

実験手法

X-RAY DIFFRACTION (3.59 Å)