5ETC

Structure of inactive MAPK14 with ordered Activation Loop

Summary for 5ETC

• Entry DOI: 10.2210/pdb5etc/pdb
• Descriptor: Mitogen-activated protein kinase 14, SULFATE ION (3 entities in total)
• Functional Keywords: mapk14, inactive kinase, activation loop, mapk, transferase
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm: Q16539
• Total number of polymer chains: 1
• Total formula weight: 41812.73

Authors

Kapp, U.,Pellegrini, E.,Bowler, M.W. (deposition date: 2015-11-17, release date: 2016-01-20, Last modification date: 2023-09-27)

Primary citation

Juyoux, P.,Galdadas, I.,Gobbo, D.,von Velsen, J.,Pelosse, M.,Tully, M.,Vadas, O.,Gervasio, F.L.,Pellegrini, E.,Bowler, M.W.
Architecture of the MKK6-p38 alpha complex defines the basis of MAPK specificity and activation.
Science, 381:1217-1225, 2023

PubMed Abstract: The mitogen-activated protein kinase (MAPK) p38α is a central component of signaling in inflammation and the immune response and is, therefore, an important drug target. Little is known about the molecular mechanism of its activation by double phosphorylation from MAPK kinases (MAP2Ks), because of the challenge of trapping a transient and dynamic heterokinase complex. We applied a multidisciplinary approach to generate a structural model of p38α in complex with its MAP2K, MKK6, and to understand the activation mechanism. Integrating cryo-electron microscopy with molecular dynamics simulations, hydrogen-deuterium exchange mass spectrometry, and experiments in cells, we demonstrate a dynamic, multistep phosphorylation mechanism, identify catalytically relevant interactions, and show that MAP2K-disordered amino termini determine pathway specificity. Our work captures a fundamental step of cell signaling: a kinase phosphorylating its downstream target kinase.

PubMed: 37708276
DOI: 10.1126/science.add7859

Experimental method

X-RAY DIFFRACTION (2.422 Å)