High resolution x-ray crystal structure of isotope-labeled ester-insulin

Summary for 5EN9

DescriptorInsulin chain A, Insulin chain B (3 entities in total)
Functional Keywordsisotope-labeled, ester-insulin, chemical protein synthesis, spontaneous resolution, hormone
Biological sourceHomo sapiens (Human)
Cellular locationSecreted P01308 P01308
Total number of polymer chains2
Total molecular weight5817.65
Mandal, K.,Dhayalan, B.,Avital-Shmilovici, M.,Tokmakoff, A.,Kent, S.B.H. (deposition date: 2015-11-09, release date: 2016-01-13, Last modification date: 2017-09-20)
Primary citation
Mandal, K.,Dhayalan, B.,Avital-Shmilovici, M.,Tokmakoff, A.,Kent, S.B.
Crystallization of Enantiomerically Pure Proteins from Quasi-Racemic Mixtures: Structure Determination by X-Ray Diffraction of Isotope-Labeled Ester Insulin and Human Insulin.
Chembiochem, 17:421-425, 2016
PubMed: 26707939 (PDB entries with the same primary citation)
DOI: 10.1002/cbic.201500600
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.1773009.8%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution