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5ELJ

Isoform-specific inhibition of SUMO-dependent protein-protein interactions

5ELJ の概要
エントリーDOI10.2210/pdb5elj/pdb
分子名称SUMO-Affirmer-S2D5, Small ubiquitin-related modifier 1 (3 entities in total)
機能のキーワードubiquitin, sumoylation, signaling protein
由来する生物種synthetic construct
詳細
細胞内の位置Nucleus membrane: P63165
タンパク質・核酸の鎖数2
化学式量合計22938.19
構造登録者
主引用文献Hughes, D.J.,Tiede, C.,Penswick, N.,Tang, A.A.,Trinh, C.H.,Mandal, U.,Zajac, K.Z.,Gaule, T.,Howell, G.,Edwards, T.A.,Duan, J.,Feyfant, E.,McPherson, M.J.,Tomlinson, D.C.,Whitehouse, A.
Generation of specific inhibitors of SUMO-1- and SUMO-2/3-mediated protein-protein interactions using Affimer (Adhiron) technology.
Sci Signal, 10:-, 2017
Cited by
PubMed Abstract: Because protein-protein interactions underpin most biological processes, developing tools that target them to understand their function or to inform the development of therapeutics is an important task. SUMOylation is the posttranslational covalent attachment of proteins in the SUMO family (SUMO-1, SUMO-2, or SUMO-3), and it regulates numerous cellular pathways. SUMOylated proteins are recognized by proteins with SUMO-interaction motifs (SIMs) that facilitate noncovalent interactions with SUMO. We describe the use of the Affimer system of peptide display for the rapid isolation of synthetic binding proteins that inhibit SUMO-dependent protein-protein interactions mediated by SIMs both in vitro and in cells. Crucially, these synthetic proteins did not prevent SUMO conjugation either in vitro or in cell-based systems, enabling the specific analysis of SUMO-mediated protein-protein interactions. Furthermore, through structural analysis and molecular modeling, we explored the molecular mechanisms that may underlie their specificity in interfering with either SUMO-1-mediated interactions or interactions mediated by either SUMO-2 or SUMO-3. Not only will these reagents enable investigation of the biological roles of SUMOylation, but the Affimer technology used to generate these synthetic binding proteins could also be exploited to design or validate reagents or therapeutics that target other protein-protein interactions.
PubMed: 29138295
DOI: 10.1126/scisignal.aaj2005
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.983 Å)
構造検証レポート
Validation report summary of 5elj
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-12-18に公開中

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