5ELH

Crystal structure of mouse Unkempt zinc fingers 1-3 (ZnF1-3), bound to RNA

Summary for 5ELH

• Entry DOI: 10.2210/pdb5elh/pdb
• Related: 5ELK
• Descriptor: RING finger protein unkempt homolog, RNA (5'-R(*UP*UP*AP*UP*U)-3'), ZINC ION, ... (5 entities in total)
• Functional Keywords: unkempt, rna-binding protein, ccch zinc fingers, rna binding protein-rna complex, rna binding protein/rna
• Biological source: Mus musculus (Mouse); More
• Total number of polymer chains: 3
• Total formula weight: 36954.52

Authors

Teplova, M.,Murn, J.,Zarnack, K.,Shi, Y.,Patel, D.J. (deposition date: 2015-11-04, release date: 2015-12-09, Last modification date: 2024-03-06)

Primary citation

Murn, J.,Teplova, M.,Zarnack, K.,Shi, Y.,Patel, D.J.
Recognition of distinct RNA motifs by the clustered CCCH zinc fingers of neuronal protein Unkempt.
Nat.Struct.Mol.Biol., 23:16-23, 2016

PubMed Abstract: Unkempt is an evolutionarily conserved RNA-binding protein that regulates translation of its target genes and is required for the establishment of the early bipolar neuronal morphology. Here we determined the X-ray crystal structure of mouse Unkempt and show that its six CCCH zinc fingers (ZnFs) form two compact clusters, ZnF1-3 and ZnF4-6, that recognize distinct trinucleotide RNA substrates. Both ZnF clusters adopt a similar overall topology and use distinct recognition principles to target specific RNA sequences. Structure-guided point mutations reduce the RNA binding affinity of Unkempt both in vitro and in vivo, ablate Unkempt's translational control and impair the ability of Unkempt to induce a bipolar cellular morphology. Our study unravels a new mode of RNA sequence recognition by clusters of CCCH ZnFs that is critical for post-transcriptional control of neuronal morphology.

PubMed: 26641712
DOI: 10.1038/nsmb.3140

Experimental method

X-RAY DIFFRACTION (1.8 Å)