5EJ6

EcMenD-ThDP-Mn2+ complex soaked with 2-ketoglutarate for 2min then soaked with isochorismate for 2 min

Summary for 5EJ6

• Entry DOI: 10.2210/pdb5ej6/pdb
• Related: 5EJ4 5EJ5 5EJ7 5EJ8 5EJ9 5EJA 5EJM
• Descriptor: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase, (4S)-4-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3lambda~5~-thiazol-2-yl}-4-hydroxybutanoic acid, MANGANESE (II) ION, ... (4 entities in total)
• Functional Keywords: post-decarboxylation intermediate, transferase
• Biological source: Escherichia coli K12
• Total number of polymer chains: 8
• Total formula weight: 496128.98

Authors

Song, H.G.,Dong, C.,Chen, Y.Z.,Sun, Y.R.,Guo, Z.H. (deposition date: 2015-11-01, release date: 2016-06-01, Last modification date: 2024-03-20)

• Primary citation: Song, H.G.,Dong, C.,Qin, M.M.,Chen, Y.Z.,Sun, Y.R.,Liu, J.J.,Chan, W.,Guo, Z.H.; A Thiamine-Dependent Enzyme Utilizes an Active Tetrahedral Intermediate in Vitamin K Biosynthesis; J.Am.Chem.Soc., 138:7244-7247, 2016; PubMed: 27213829; DOI: 10.1021/jacs.6b03437

Experimental method

X-RAY DIFFRACTION (2.243 Å)