5EFW

Crystal structure of LOV2-Zdk1 - the complex of oat LOV2 and the affibody protein Zdark1

Summary for 5EFW

Entry DOI	10.2210/pdb5efw/pdb
Related	1LP1 2V0U
Descriptor	NPH1-1, Z-dark, a small protein based on the Z domain affibody, FLAVIN MONONUCLEOTIDE, ... (5 entities in total)
Functional Keywords	lov domain, photoreceptor, affibody, optogenetic tool, signaling protein
Biological source	Avena sativa (Oat) More
Total number of polymer chains	3
Total formula weight	30795.12
Authors	Winkler, A.,Wang, H.,Hartmann, E.,Hahn, K.,Schlichting, I. (deposition date: 2015-10-26, release date: 2016-07-20, Last modification date: 2024-01-10)
Primary citation	Wang, H.,Vilela, M.,Winkler, A.,Tarnawski, M.,Schlichting, I.,Yumerefendi, H.,Kuhlman, B.,Liu, R.,Danuser, G.,Hahn, K.M. LOVTRAP: an optogenetic system for photoinduced protein dissociation. Nat.Methods, 13:755-758, 2016 Cited by PubMed Abstract: LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins. PubMed: 27427858 DOI: 10.1038/nmeth.3926 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.1 Å)

Structure validation