5ED4

Structure of a PhoP-DNA complex

5ED4 の概要

• エントリーDOI: 10.2210/pdb5ed4/pdb
• 分子名称: Response regulator, DNA (26-MER), CALCIUM ION, ... (7 entities in total)
• 機能のキーワード: protein-dna complex, winged helix-turn-helix, direct repeat, tandem dimer, transcription-dna complex, transcription/dna
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 143959.57

構造登録者

Wang, S. (登録日: 2015-10-20, 公開日: 2016-04-27, 最終更新日: 2023-09-27)

主引用文献

He, X.,Wang, L.,Wang, S.
Structural basis of DNA sequence recognition by the response regulator PhoP in Mycobacterium tuberculosis.
Sci Rep, 6:24442-24442, 2016

PubMed Abstract: The transcriptional regulator PhoP is an essential virulence factor in Mycobacterium tuberculosis, and it presents a target for the development of new anti-tuberculosis drugs and attenuated tuberculosis vaccine strains. PhoP binds to DNA as a highly cooperative dimer by recognizing direct repeats of 7-bp motifs with a 4-bp spacer. To elucidate the PhoP-DNA binding mechanism, we determined the crystal structure of the PhoP-DNA complex. The structure revealed a tandem PhoP dimer that bound to the direct repeat. The surprising tandem arrangement of the receiver domains allowed the four domains of the PhoP dimer to form a compact structure, accounting for the strict requirement of a 4-bp spacer and the highly cooperative binding of the dimer. The PhoP-DNA interactions exclusively involved the effector domain. The sequence-recognition helix made contact with the bases of the 7-bp motif in the major groove, and the wing interacted with the adjacent minor groove. The structure provides a starting point for the elucidation of the mechanism by which PhoP regulates the virulence of M. tuberculosis and guides the design of screening platforms for PhoP inhibitors.

PubMed: 27079268
DOI: 10.1038/srep24442

実験手法

X-RAY DIFFRACTION (2.4 Å)