5E9S

Crystal structure of substrate-bound glutamate transporter homologue GltTk

5E9S の概要

• エントリーDOI: 10.2210/pdb5e9s/pdb
• 関連するPDBエントリー: 5DWY
• 分子名称: Proton/glutamate symporter, SDF family, ASPARTIC ACID, SODIUM ION, ... (8 entities in total)
• 機能のキーワード: amino acid transporter, aspartate transport, glutamate transport homologue, transport protein, membrane protein
• 由来する生物種: Thermococcus kodakarensis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 149742.77

構造登録者

Guskov, A.,Slotboom, D.J. (登録日: 2015-10-15, 公開日: 2016-09-28, 最終更新日: 2024-01-10)

主引用文献

Guskov, A.,Jensen, S.,Faustino, I.,Marrink, S.J.,Slotboom, D.J.
Coupled binding mechanism of three sodium ions and aspartate in the glutamate transporter homologue GltTk.
Nat Commun, 7:13420-13420, 2016

PubMed Abstract: Glutamate transporters catalyse the thermodynamically unfavourable transport of anionic amino acids across the cell membrane by coupling it to the downhill transport of cations. This coupling mechanism is still poorly understood, in part because the available crystal structures of these transporters are of relatively low resolution. Here we solve crystal structures of the archaeal transporter Glt in the presence and absence of aspartate and use molecular dynamics simulations and binding assays to show how strict coupling between the binding of three sodium ions and aspartate takes place.

PubMed: 27830699
DOI: 10.1038/ncomms13420

実験手法

X-RAY DIFFRACTION (2.8 Å)