5DWY
Crystal structure of a substrate-free glutamate transporter homologue GltTk
Summary for 5DWY
| Entry DOI | 10.2210/pdb5dwy/pdb |
| Related | 4KY0 |
| Descriptor | Proton/glutamate symporter, SDF family, TRIETHYLENE GLYCOL, DI(HYDROXYETHYL)ETHER, ... (5 entities in total) |
| Functional Keywords | amino acid transporter, aspartate transport, glutamate transport homologue, transport protein, membrane protein |
| Biological source | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) |
| Total number of polymer chains | 3 |
| Total formula weight | 144733.49 |
| Authors | Guskov, A.,Slotboom, D.J. (deposition date: 2015-09-23, release date: 2016-09-28, Last modification date: 2024-01-10) |
| Primary citation | Guskov, A.,Jensen, S.,Faustino, I.,Marrink, S.J.,Slotboom, D.J. Coupled binding mechanism of three sodium ions and aspartate in the glutamate transporter homologue GltTk. Nat Commun, 7:13420-13420, 2016 Cited by PubMed Abstract: Glutamate transporters catalyse the thermodynamically unfavourable transport of anionic amino acids across the cell membrane by coupling it to the downhill transport of cations. This coupling mechanism is still poorly understood, in part because the available crystal structures of these transporters are of relatively low resolution. Here we solve crystal structures of the archaeal transporter Glt in the presence and absence of aspartate and use molecular dynamics simulations and binding assays to show how strict coupling between the binding of three sodium ions and aspartate takes place. PubMed: 27830699DOI: 10.1038/ncomms13420 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.7 Å) |
Structure validation
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