5E8L
Crystal structure of geranylgeranyl pyrophosphate synthase 11 from Arabidopsis thaliana
Summary for 5E8L
| Entry DOI | 10.2210/pdb5e8l/pdb |
| Related | 5E8H 5E8K |
| Descriptor | Heterodimeric geranylgeranyl pyrophosphate synthase large subunit 1, chloroplastic (1 entity in total) |
| Functional Keywords | atppps2, transferase |
| Biological source | Arabidopsis thaliana (Mouse-ear cress) |
| Total number of polymer chains | 2 |
| Total formula weight | 67201.19 |
| Authors | |
| Primary citation | Wang, C.,Chen, Q.,Fan, D.,Li, J.,Wang, G.,Zhang, P. Structural Analyses of Short-Chain Prenyltransferases Identify an Evolutionarily Conserved GFPPS Clade in Brassicaceae Plants. Mol Plant, 9:195-204, 2016 Cited by PubMed Abstract: Terpenoids are the largest and most diverse class of plant-specialized metabolites, which function in diverse physiological processes during plant development. In the biosynthesis of plant terpenoids, short-chain prenyltransferases (SC-PTs), together with terpene synthases (TPSs), play critical roles in determining terpenoid diversity. SC-PTs biosynthesize prenyl pyrophosphates with different chain lengths, and these compounds are the direct precursors of terpenoids. Arabidopsis thaliana possesses a subgroup of SC-PTs whose functions are not clearly known. In this study, we focus on 10 geranylgeranyl pyrophosphate synthase-like [GGPPSL] proteins, which are commonly thought to produce GGPP [C20]. We found that a subset of members of the Arabidopsis GGPPSL gene family have undergone neo-functionalization: GGPPSL6, 7, 9, and 10 mainly have geranylfarnesyl pyrophosphate synthase activity (C25; renamed AtGFPPS1, 2, 3, and 4), and GGPPSL8 produces even longer chain prenyl pyrophosphate (≥ C30; renamed polyprenyl pyrophosphate synthase 2, AtPPPS2). By solving the crystal structures of AtGFPPS2, AtPPPS2, and AtGGPPS11, we reveal the product chain-length determination mechanism of SC-PTs and interpret it as a "three floors" model. Using this model, we identified a novel GFPPS clade distributed in Brassicaceae plants and found that the GFPPS gene typically occurs in tandem with a gene encoding a TPS, forming a GFPPS-TPS gene cluster. PubMed: 26537048DOI: 10.1016/j.molp.2015.10.010 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.807 Å) |
Structure validation
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