5E8L
Crystal structure of geranylgeranyl pyrophosphate synthase 11 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-10-08 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.111, 97.831, 109.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.145 - 2.807 |
R-factor | 0.1905 |
Rwork | 0.188 |
R-free | 0.23650 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.709 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 16227 |
<I/σ(I)> | 13.7 |
Completeness [%] | 95.6 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.4 M Magnesium formate dihydrate, 0.1 M sodium acetate trihydrate, pH 4.6 |