5E54

Two apo structures of the adenine riboswitch aptamer domain determined using an X-ray free electron laser

Summary for 5E54

DescriptorVibrio vulnificus strain 93U204 chromosome II, adenine riboswitch aptamer domain, MAGNESIUM ION (3 entities in total)
Functional Keywordsadenine riboswitch, purine riboswitch, apo, ligand-free, rna, gene regulation, x-ray free electron laser
Biological sourceVibrio vulnificus
Total number of polymer chains2
Total molecular weight45471.99
Authors
Primary citation
Stagno, J.R.,Liu, Y.,Bhandari, Y.R.,Conrad, C.E.,Panja, S.,Swain, M.,Fan, L.,Nelson, G.,Li, C.,Wendel, D.R.,White, T.A.,Coe, J.D.,Wiedorn, M.O.,Knoska, J.,Oberthuer, D.,Tuckey, R.A.,Yu, P.,Dyba, M.,Tarasov, S.G.,Weierstall, U.,Grant, T.D.,Schwieters, C.D.,Zhang, J.,Ferre-D'Amare, A.R.,Fromme, P.,Draper, D.E.,Liang, M.,Hunter, M.S.,Boutet, S.,Tan, K.,Zuo, X.,Ji, X.,Barty, A.,Zatsepin, N.A.,Chapman, H.N.,Spence, J.C.,Woodson, S.A.,Wang, Y.X.
Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography.
Nature, 541:242-246, 2017
PubMed: 27841871 (PDB entries with the same primary citation)
DOI: 10.1038/nature20599
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.3 Å)
?

Structure validation

RfreeClashscoreRSRZ outliersRNA backbone0.254212.8%0.57MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload