5E27

The structure of Resuscitation Promoting Factor B from M. tuberculosis reveals unexpected ubiquitin-like domains

Summary for 5E27

• Entry DOI: 10.2210/pdb5e27/pdb
• Descriptor: Resuscitation-promoting factor RpfB (2 entities in total)
• Functional Keywords: cell wall, peptidoglycan, tuberculosis, cell adhesion
• Biological source: Mycobacterium tuberculosis
• Total number of polymer chains: 2
• Total formula weight: 51806.61

Authors

Ruggiero, A.,Squeglia, F.,Romano, M.,Vitagliano, L.,De Simone, A.,Berisio, R. (deposition date: 2015-09-30, release date: 2015-11-18, Last modification date: 2024-11-06)

Primary citation

Ruggiero, A.,Squeglia, F.,Romano, M.,Vitagliano, L.,De Simone, A.,Berisio, R.
The structure of Resuscitation promoting factor B from M. tuberculosis reveals unexpected ubiquitin-like domains.
Biochim.Biophys.Acta, 1860:445-451, 2015

PubMed Abstract: RpfB is a key factor in resuscitation from dormancy of Mycobacterium tuberculosis. This protein is a cell-wall glycosidase, which cleaves cell-wall peptidoglycan. RpfB is structurally complex and is composed of three types of domains, including a catalytic, a G5 and three DUF348 domains. Structural information is currently limited to a portion of the protein including only the catalytic and G5 domains. To gain insights into the structure and function of all domains we have undertaken structural investigations on a large protein fragment containing all three types of domains that constitute RpfB (RpfB3D).

PubMed: 26549874
DOI: 10.1016/j.bbagen.2015.11.001

Experimental method

X-RAY DIFFRACTION (2.6 Å)