5DV7

Crystal Structure of NF90 tandem dsRBDs with dsRNA

5DV7 の概要

• エントリーDOI: 10.2210/pdb5dv7/pdb
• 分子名称: RNA (5'-R(*CP*CP*AP*GP*CP*AP*UP*UP*AP*UP*GP*AP*AP*AP*GP*UP*GP*A)-3'), RNA (5'-R(*UP*CP*AP*CP*UP*UP*UP*CP*AP*UP*AP*AP*UP*GP*CP*UP*GP*G)-3'), Interleukin enhancer-binding factor 3, ... (4 entities in total)
• 機能のキーワード: double stranded rna binding domains, dsrm, dsrbds, dsrna, nf90, ilf3, drbp76, rna binding protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• 細胞内の位置: Nucleus, nucleolus : Q9Z1X4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 87524.56

構造登録者

Jayachandran, U.,Grey, H.,Cook, A.G. (登録日: 2015-09-21, 公開日: 2016-01-13, 最終更新日: 2024-01-10)

主引用文献

Jayachandran, U.,Grey, H.,Cook, A.G.
Nuclear factor 90 uses an ADAR2-like binding mode to recognize specific bases in dsRNA.
Nucleic Acids Res., 44:1924-1936, 2016

PubMed Abstract: Nuclear factors 90 and 45 (NF90 and NF45) form a protein complex involved in the post-transcriptional control of many genes in vertebrates. NF90 is a member of the dsRNA binding domain (dsRBD) family of proteins. RNA binding partners identified so far include elements in 3' untranslated regions of specific mRNAs and several non-coding RNAs. In NF90, a tandem pair of dsRBDs separated by a natively unstructured segment confers dsRNA binding activity. We determined a crystal structure of the tandem dsRBDs of NF90 in complex with a synthetic dsRNA. This complex shows surprising similarity to the tandem dsRBDs from an adenosine-to-inosine editing enzyme, ADAR2 in complex with a substrate RNA. Residues involved in unusual base-specific recognition in the minor groove of dsRNA are conserved between NF90 and ADAR2. These data suggest that, like ADAR2, underlying sequences in dsRNA may influence how NF90 recognizes its target RNAs.

PubMed: 26712564
DOI: 10.1093/nar/gkv1508

実験手法

X-RAY DIFFRACTION (3.5 Å)