5DQO

Crystal structure of Y347F mutant of human primase p58 iron-sulfur cluster domain

5DQO の概要

• エントリーDOI: 10.2210/pdb5dqo/pdb
• 関連するPDBエントリー: 3L9Q
• 分子名称: DNA primase large subunit, IRON/SULFUR CLUSTER (3 entities in total)
• 機能のキーワード: iron-sulfur cluster, dna priming, replication
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 92030.36

構造登録者

Salay, L.E.,Thompson, M.K.,Chazin, W.J. (登録日: 2015-09-15, 公開日: 2016-09-21, 最終更新日: 2023-09-27)

主引用文献

O'Brien, E.,Holt, M.E.,Thompson, M.K.,Salay, L.E.,Ehlinger, A.C.,Chazin, W.J.,Barton, J.K.
The [4Fe4S] cluster of human DNA primase functions as a redox switch using DNA charge transport.
Science, 355:-, 2017

PubMed Abstract: DNA charge transport chemistry offers a means of long-range, rapid redox signaling. We demonstrate that the [4Fe4S] cluster in human DNA primase can make use of this chemistry to coordinate the first steps of DNA synthesis. Using DNA electrochemistry, we found that a change in oxidation state of the [4Fe4S] cluster acts as a switch for DNA binding. Single-atom mutations that inhibit this charge transfer hinder primase initiation without affecting primase structure or polymerization. Generating a single base mismatch in the growing primer duplex, which attenuates DNA charge transport, inhibits primer truncation. Thus, redox signaling by [4Fe4S] clusters using DNA charge transport regulates primase binding to DNA and illustrates chemistry that may efficiently drive substrate handoff between polymerases during DNA replication.

PubMed: 28232525
DOI: 10.1126/science.aag1789

実験手法

X-RAY DIFFRACTION (2.3 Å)