5DM9

XFEL structure of hen egg-white lysozyme solved using a droplet injector at SACLA

Summary for 5DM9

• Entry DOI: 10.2210/pdb5dm9/pdb
• Descriptor: Lysozyme C, CHLORIDE ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: lysozyme, serial femtosecond crystallography, hydrolase
• Biological source: Gallus gallus (Chicken)
• Cellular location: Secreted: P00698
• Total number of polymer chains: 1
• Total formula weight: 14425.06

Authors

Kobayashi, J.,Nango, E. (deposition date: 2015-09-08, release date: 2016-04-13, Last modification date: 2024-10-16)

Primary citation

Mafune, F.,Miyajima, K.,Tono, K.,Takeda, Y.,Kohno, J.Y.,Miyauchi, N.,Kobayashi, J.,Joti, Y.,Nango, E.,Iwata, S.,Yabashi, M.
Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography.
Acta Crystallogr D Struct Biol, 72:520-523, 2016

PubMed Abstract: A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 µm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 Å from <0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.

PubMed: 27050131
DOI: 10.1107/S2059798316001480

Experimental method

X-RAY DIFFRACTION (2.3 Å)