5DHK
Nicotiana tabacum 5-epi-aristolochene synthase mutant W273E - alkylated
Summary for 5DHK
| Entry DOI | 10.2210/pdb5dhk/pdb |
| Related | 5DHI |
| Descriptor | 5-epi-aristolochene synthase, MAGNESIUM ION, FARNESYL, ... (4 entities in total) |
| Functional Keywords | sesquiterpene synthase, 5-epi-aristolochene synthase, farnesylation, active site alkylation, lyase |
| Biological source | Nicotiana tabacum (Common tobacco) |
| Total number of polymer chains | 1 |
| Total formula weight | 61989.09 |
| Authors | Noel, J.P.,Kersten, R.K. (deposition date: 2015-08-31, release date: 2015-09-16, Last modification date: 2023-09-27) |
| Primary citation | Kersten, R.D.,Diedrich, J.K.,Yates, J.R.,Noel, J.P. Mechanism-Based Post-Translational Modification and Inactivation in Terpene Synthases. Acs Chem.Biol., 10:2501-2511, 2015 Cited by PubMed Abstract: Terpenes are ubiquitous natural chemicals with diverse biological functions spanning all three domains of life. In specialized metabolism, the active sites of terpene synthases (TPSs) evolve in shape and reactivity to direct the biosynthesis of a myriad of chemotypes for organismal fitness. As most terpene biosynthesis mechanistically involves highly reactive carbocationic intermediates, the protein surfaces catalyzing these cascade reactions possess reactive regions possibly prone to premature carbocation capture and potentially enzyme inactivation. Here, we show using proteomic and X-ray crystallographic analyses that cationic intermediates undergo capture by conserved active site residues leading to inhibitory self-alkylation. Moreover, the level of cation-mediated inactivation increases with mutation of the active site, upon changes in the size and structure of isoprenoid diphosphate substrates, and alongside increases in reaction temperatures. TPSs that individually synthesize multiple products are less prone to self-alkylation then TPSs possessing relatively high product specificity. In total, the results presented suggest that mechanism-based alkylation represents an overlooked mechanistic pressure during the evolution of cation-derived terpene biosynthesis. PubMed: 26378620DOI: 10.1021/acschembio.5b00539 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.43 Å) |
Structure validation
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