5DGY

Crystal structure of rhodopsin bound to visual arrestin

5DGY の概要

• エントリーDOI: 10.2210/pdb5dgy/pdb
• 関連するPDBエントリー: 4ZWJ
• 分子名称: Endolysin,Rhodopsin,S-arrestin (1 entity in total)
• 機能のキーワード: gpcr, rhodopsin, arrestin, signaling protein
• 由来する生物種: Enterobacteria phage T4; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 403444.41

構造登録者

Zhou, X.E.,Gao, X.,Kang, Y.,He, Y.,de Waal, P.W.,Suino-Powell, K.M.,Wang, M.,Melcher, K.,Xu, H.E. (登録日: 2015-08-28, 公開日: 2016-03-23, 最終更新日: 2024-10-16)

主引用文献

Zhou, X.E.,Gao, X.,Barty, A.,Kang, Y.,He, Y.,Liu, W.,Ishchenko, A.,White, T.A.,Yefanov, O.,Han, G.W.,Xu, Q.,de Waal, P.W.,Suino-Powell, K.M.,Boutet, S.,Williams, G.J.,Wang, M.,Li, D.,Caffrey, M.,Chapman, H.N.,Spence, J.C.,Fromme, P.,Weierstall, U.,Stevens, R.C.,Cherezov, V.,Melcher, K.,Xu, H.E.
X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex.
Sci Data, 3:160021-160021, 2016

PubMed Abstract: Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes.

PubMed: 27070998
DOI: 10.1038/sdata.2016.21

実験手法

X-RAY DIFFRACTION (7.7 Å)