5DGK

SCCmec type IV Cch - active helicase

5DGK の概要

• エントリーDOI: 10.2210/pdb5dgk/pdb
• 分子名称: Active helicase, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: active ring shaped helicase, replication
• 由来する生物種: Staphylococcus aureus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 137690.85

構造登録者

Rice, P.A.,Mir-Sanchis, I. (登録日: 2015-08-27, 公開日: 2016-08-24, 最終更新日: 2024-10-30)

主引用文献

Mir-Sanchis, I.,Roman, C.A.,Misiura, A.,Pigli, Y.Z.,Boyle-Vavra, S.,Rice, P.A.
Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative.
Nat.Struct.Mol.Biol., 23:891-898, 2016

PubMed Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is a public-health threat worldwide. Although the mobile genomic island responsible for this phenotype, staphylococcal cassette chromosome (SCC), has been thought to be nonreplicative, we predicted DNA-replication-related functions for some of the conserved proteins encoded by SCC. We show that one of these, Cch, is homologous to the self-loading initiator helicases of an unrelated family of genomic islands, that it is an active 3'-to-5' helicase and that the adjacent ORF encodes a single-stranded DNA-binding protein. Our 2.9-Å crystal structure of intact Cch shows that it forms a hexameric ring. Cch, like the archaeal and eukaryotic MCM-family replicative helicases, belongs to the pre-sensor II insert clade of AAA+ ATPases. Additionally, we found that SCC elements are part of a broader family of mobile elements, all of which encode a replication initiator upstream of their recombinases. Replication after excision would enhance the efficiency of horizontal gene transfer.

PubMed: 27571176
DOI: 10.1038/nsmb.3286

実験手法

X-RAY DIFFRACTION (2.895 Å)