5DF1

Iridoid synthase from Catharanthus roseus - ternary complex with NADP+ and geranic acid

5DF1 の概要

• エントリーDOI: 10.2210/pdb5df1/pdb
• 分子名称: Iridoid synthase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, (2E)-3,7-dimethylocta-2,6-dienoic acid, ... (6 entities in total)
• 機能のキーワード: oxidoreductase, iridoid synthase, short chain dehydrogenase, nadph-dependent, catharanthus roseus
• 由来する生物種: Catharanthus roseus (Madagascar periwinkle)
• 細胞内の位置: Cytoplasm, cytosol : K7WDL7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85051.78

構造登録者

Caputi, L.,Kries, H.,Stevenson, C.E.M.,Kamileen, M.O.,Sherden, N.H.,Geu-Flores, F.,Lawson, D.M.,O'Connor, S.E. (登録日: 2015-08-26, 公開日: 2015-10-28, 最終更新日: 2024-01-10)

主引用文献

Kries, H.,Caputi, L.,Stevenson, C.E.,Kamileen, M.O.,Sherden, N.H.,Geu-Flores, F.,Lawson, D.M.,O'Connor, S.E.
Structural determinants of reductive terpene cyclization in iridoid biosynthesis.
Nat.Chem.Biol., 12:6-8, 2016

PubMed Abstract: The carbon skeleton of ecologically and pharmacologically important iridoid monoterpenes is formed in a reductive cyclization reaction unrelated to canonical terpene cyclization. Here we report the crystal structure of the recently discovered iridoid cyclase (from Catharanthus roseus) bound to a mechanism-inspired inhibitor that illuminates substrate binding and catalytic function of the enzyme. Key features that distinguish iridoid synthase from its close homolog progesterone 5β-reductase are highlighted.

PubMed: 26551396
DOI: 10.1038/nchembio.1955

実験手法

X-RAY DIFFRACTION (1.75 Å)