5DC3

Complex of yeast 80S ribosome with non-modified eIF5A

This is a non-PDB format compatible entry.

Summary for 5DC3

• Entry DOI: 10.2210/pdb5dc3/pdb
• Descriptor: 18S ribosomal RNA, 40S ribosomal protein S8-A, 40S ribosomal protein S9-A, ... (86 entities in total)
• Functional Keywords: ribosome, complex, eif5a
• Biological source: Saccharomyces cerevisiae S288c; More
• Total number of polymer chains: 163
• Total formula weight: 6288663.58

Authors

Melnikov, S.,Mailliot, J.,Shin, B.-S.,Rigger, L.,Yusupova, G.,Micura, R.,Dever, T.E.,Yusupov, M. (deposition date: 2015-08-23, release date: 2016-06-01, Last modification date: 2024-01-10)

Primary citation

Melnikov, S.,Mailliot, J.,Shin, B.S.,Rigger, L.,Yusupova, G.,Micura, R.,Dever, T.E.,Yusupov, M.
Crystal Structure of Hypusine-Containing Translation Factor eIF5A Bound to a Rotated Eukaryotic Ribosome.
J.Mol.Biol., 428:3570-3576, 2016

PubMed Abstract: Eukaryotic translation initiation factor eIF5A promotes protein synthesis by resolving polyproline-induced ribosomal stalling. Here, we report a 3.25-Å resolution crystal structure of eIF5A bound to the yeast 80S ribosome. The structure reveals a previously unseen conformation of an eIF5A-ribosome complex and highlights a possible functional link between conformational changes of the ribosome during protein synthesis and the eIF5A-ribosome association.

PubMed: 27196944
DOI: 10.1016/j.jmb.2016.05.011

Experimental method

X-RAY DIFFRACTION (3.25 Å)