5D9I

SV40 Large T antigen origin binding domain bound to artificial DNA fork

5D9I の概要

• エントリーDOI: 10.2210/pdb5d9i/pdb
• 分子名称: Large T antigen, DNA (5'-D(*GP*AP*GP*GP*AP*GP*GP*CP*TP*TP*TP*TP*TP*TP*GP*GP*AP*GP*GP*CP*C)-3'), DNA (5'-D(*AP*CP*TP*CP*CP*TP*CP*CP*GP*AP*AP*AP*AP*AP*AP*CP*CP*TP*CP*CP*GP*GP*A)-3'), ... (7 entities in total)
• 機能のキーワード: origin binding domain, replication, sv40, replication-dna complex, replication/dna
• 由来する生物種: Simian virus 40 (SV40); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 44758.59

構造登録者

Meinke, G.,Bohm, A.,Bullock, P.A. (登録日: 2015-08-18, 公開日: 2015-09-09, 最終更新日: 2024-10-23)

主引用文献

Meinke, G.,Phelan, P.J.,Fradet-Turcotte, A.,Bohm, A.,Archambault, J.,Bullock, P.A.
Structure-based analysis of the interaction between the simian virus 40 T-antigen origin binding domain and single-stranded DNA.
J. Virol., 85:818-827, 2011

PubMed Abstract: The origin-binding domain (OBD) of simian virus 40 (SV40) large T-antigen (T-Ag) is essential for many of T-Ag's interactions with DNA. Nevertheless, many important issues related to DNA binding, for example, how single-stranded DNA (ssDNA) transits along the T-Ag OBD, have yet to be established. Therefore, X-ray crystallography was used to determine the costructure of the T-Ag OBD bound to DNA substrates such as the single-stranded region of a forked oligonucleotide. A second structure of the T-Ag OBD crystallized in the presence of poly(dT)(12) is also reported. To test the conclusions derived from these structures, residues identified as being involved in binding to ssDNA by crystallography or by an earlier nuclear magnetic resonance study were mutated, and their binding to DNA was characterized via fluorescence anisotropy. In addition, these mutations were introduced into full-length T-Ag, and these mutants were tested for their ability to support replication. When considered in terms of additional homology-based sequence alignments, our studies refine our understanding of how the T-Ag OBDs encoded by the polyomavirus family interact with ssDNA, a critical step during the initiation of DNA replication.

PubMed: 20980496
DOI: 10.1128/JVI.01738-10

実験手法

X-RAY DIFFRACTION (1.7 Å)