5CY1

Tn3 resolvase - site III complex crystal form I

> Summary

Summary for 5CY1

Related5CY2
DescriptorTransposon Tn3 resolvase/DNA Complex
Functional Keywordsdna recombinase repressor dna binding protein, hydrolase, ligase-dna complex, ligase/dna
Biological sourceEscherichia coli
Total number of polymer chains4
Total molecular weight61545.17
Authors
Montano, S.P.,Rice, P.A. (deposition date: 2015-07-30, release date: 2017-01-11)
Primary citation
Montano, S.P.,Rice, P.A.
Tn3 resolvase - accessory site complexes: DNA geometry dictates complex geometry
To Be Published,
Experimental method
X-RAY DIFFRACTION (3.4 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.305500.3%6.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5cy1
no rotation
Molmil generated image of 5cy1
rotated about x axis by 90°
Molmil generated image of 5cy1
rotated about y axis by 90°

> Structural details

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
A, BTransposon Tn3 resolvasepolymer19221551.62
UniProt (P0ADI2)
Pfam (PF00239)
Pfam (PF02796)
Escherichia coli
CDNA (30-MER)polymer309234.01
DDNA (30-MER)polymer309208.01

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains4
Total molecular weight61545.2
Non-Polymers*Number of molecules0
Total molecular weight0.0
All*Total molecular weight61545.2
*Water molecules are not included.

> Experimental details

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (3.4 Å)

Cell axes77.31377.313272.277
Cell angles90.0090.0090.00
SpacegroupP 43 21 2
Resolution limits29.43 - 3.40
the highest resolution shell value3.742 - 3.400
R-factor0.2506
R-work0.24850
the highest resolution shell value0.352
R-free0.29190
the highest resolution shell value0.355
RMSD bond length0.003
RMSD bond angle0.538

Data Collection Statistics

Resolution limits100.00 - 3.40
the highest resolution shell value -
Number of reflections12184
Completeness99.9
Redundancy7.6
the highest resolution shell value6.7

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP7292

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
Annotated Information is extracted from Literature Info*

> Functional details

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Functional Information from GO Data

ChainGOidnamespacecontents
A0003677molecular_functionDNA binding
A0000150molecular_functionrecombinase activity
A0015074biological_processDNA integration
B0003677molecular_functionDNA binding
B0000150molecular_functionrecombinase activity
B0015074biological_processDNA integration
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Functional Information from PDB Data

site_idNumber of ResiduesDetails
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Functional Information from PDB atom coordinates for the "HETATM" binding sites

site_idNumber of ResiduesDetails
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Functional Information from PROSITE/UniProt

site_idNumber of ResiduesDetails
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Functional Information from SwissProt/UniProt

site_idNumber of ResiduesDetails
SWS_FT_FI11O-(5'-phospho-DNA)-serine intermediate. {ECO:0000255|PROSITE-ProRule:PRU01072}.
ChainResidueDetails
ASER10

SWS_FT_FI21H-T-H motif. {ECO:0000255}.
ChainResidueDetails
AALA161-GLU180

SWS_FT_FI31O-(5'-phospho-DNA)-serine intermediate. {ECO:0000255|PROSITE-ProRule:PRU01072}.
ChainResidueDetails
BSER10

SWS_FT_FI41H-T-H motif. {ECO:0000255}.
ChainResidueDetails
BALA161-GLU180

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Catalytic Information from CSA

site_idNumber of ResiduesDetails

> Sequence Neighbor

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