5CVS

GlgE isoform 1 from Streptomyces coelicolor E423A mutant soaked in maltoheptaose

Summary for 5CVS

• Entry DOI: 10.2210/pdb5cvs/pdb
• Related PRD ID: PRD_900030
• Descriptor: Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase 1, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: hydrolase, alpha-glucan biosynthesis, glycoside hydrolase family 13_3, transferase
• Biological source: Streptomyces coelicolor
• Total number of polymer chains: 2
• Total formula weight: 154634.32

Authors

Rashid, A.M.,Syson, K.,Koliwer-Brandl, H.,van de Weerd, R.,Stevenson, C.E.M.,Batey, S.F.D.,Miah, F.,Alber, M.,Ioerger, T.R.,Chandra, G.,Appelmelk, B.J.,Nartowski, K.P.,Khimyak, Y.Z.,Lawson, D.M.,Jacobs, W.R.,Geurtsen, J.,Kalscheuer, R.,Bornemann, S. (deposition date: 2015-07-27, release date: 2016-08-17, Last modification date: 2024-01-10)

• Primary citation: Syson, K.,Stevenson, C.E.,Miah, F.,Barclay, J.E.,Tang, M.,Gorelik, A.,Rashid, A.M.,Lawson, D.M.,Bornemann, S.; Ligand-bound structures and site-directed mutagenesis identify the acceptor and secondary binding sites of Streptomyces coelicolor maltosyltransferase GlgE.; J.Biol.Chem., 291:21531-21540, 2016; PubMed: 27531751; DOI: 10.1074/jbc.M116.748160

Experimental method

X-RAY DIFFRACTION (2.3 Å)