5CVM

USP46~ubiquitin BEA covalent complex

5CVM の概要

• エントリーDOI: 10.2210/pdb5cvm/pdb
• 関連するPDBエントリー: 5CVL 5CVN 5CVO
• 分子名称: Ubiquitin carboxyl-terminal hydrolase 46, Polyubiquitin-B, ZINC ION, ... (4 entities in total)
• 機能のキーワード: usp46 ubiquitin covalent complex, dub, deubiquitinase, hydrolase-signaling protein complex, hydrolase/signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Ubiquitin: Cytoplasm : P0CG47
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52773.17

構造登録者

Harris, S.F.,Yin, J. (登録日: 2015-07-27, 公開日: 2015-10-07, 最終更新日: 2024-11-13)

主引用文献

Yin, J.,Schoeffler, A.J.,Wickliffe, K.,Newton, K.,Starovasnik, M.A.,Dueber, E.C.,Harris, S.F.
Structural Insights into WD-Repeat 48 Activation of Ubiquitin-Specific Protease 46.
Structure, 23:2043-2054, 2015

PubMed Abstract: Protein ubiquitination patterns are an important component of cellular signaling. The WD-repeat protein WDR48 (USP1-associated factor UAF-1) stimulates activity of ubiquitin-specific proteases USP1, USP12, and USP46. To understand how WDR48 exerts its effect on the USP scaffold, we determined structures of the ternary WDR48:USP46:ubiquitin complex. WDR48 interacts with the USP46 fingers subdomain via a relatively small, highly polar surface on the top center of the WDR48 β propeller. In addition, WDR48 has a novel ancillary domain and a C-terminal SUMO-like domain encircling the USP46-bound ubiquitin. Mutation of residues involved in the WDR48:USP46 interaction abrogated both binding and deubiquitinase activity of the complex. An analogous mutation in USP1 similarly blocked WDR48-dependent activation. Our data suggest a possible mechanism of deubiquitinase stimulation via stabilization and prolonged residence time of substrate. The unprecedented mode of interaction between the USP fingers domain and the WD-repeat β propeller serves as a prototypical example for this family of deubiquitinases.

PubMed: 26388029
DOI: 10.1016/j.str.2015.08.010

実験手法

X-RAY DIFFRACTION (1.9 Å)