5CMR

Crystal Structure of Linker-Mediated Zn-bound Human H-Ferritin variant 122H-delta C-star

5CMR の概要

• エントリーDOI: 10.2210/pdb5cmr/pdb
• 関連するPDBエントリー: 5CMQ
• 分子名称: Ferritin heavy chain, ZINC ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: protein engineering, metal binding, supramolecular assembly, oxidoreductase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21461.19

構造登録者

Sontz, P.A.,Bailey, J.B.,Ahn, S.,Tezcan, F.A. (登録日: 2015-07-17, 公開日: 2015-09-09, 最終更新日: 2023-09-27)

主引用文献

Sontz, P.A.,Bailey, J.B.,Ahn, S.,Tezcan, F.A.
A Metal Organic Framework with Spherical Protein Nodes: Rational Chemical Design of 3D Protein Crystals.
J.Am.Chem.Soc., 137:11598-11601, 2015

PubMed Abstract: We describe here the construction of a three-dimensional, porous, crystalline framework formed by spherical protein nodes that assemble into a prescribed lattice arrangement through metal-organic linker-directed interactions. The octahedral iron storage enzyme, ferritin, was engineered in its C3 symmetric pores with tripodal Zn coordination sites. Dynamic light scattering and crystallographic studies established that this Zn-ferritin construct could robustly self-assemble into the desired bcc-type crystals upon coordination of a ditopic linker bearing hydroxamic acid functional groups. This system represents the first example of a ternary protein-metal-organic crystalline framework whose formation is fully dependent on each of its three components.

PubMed: 26305584
DOI: 10.1021/jacs.5b07463

実験手法

X-RAY DIFFRACTION (3.792 Å)