5CMR
Crystal Structure of Linker-Mediated Zn-bound Human H-Ferritin variant 122H-delta C-star
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 155.320, 155.320, 155.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.914 - 3.792 |
R-factor | 0.2124 |
Rwork | 0.207 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cei |
RMSD bond length | 0.005 |
RMSD bond angle | 1.067 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 109.828 | 54.914 | 4.000 |
High resolution limit [Å] | 3.792 | 11.990 | 3.790 |
Rmerge | 0.032 | 0.555 | |
Rmeas | 0.194 | ||
Rpim | 0.056 | 0.011 | 0.162 |
Total number of observations | 38132 | 1226 | 5798 |
Number of reflections | 3401 | ||
<I/σ(I)> | 12 | 35 | 5 |
Completeness [%] | 99.8 | 96.9 | 100 |
Redundancy | 11.2 | 9.1 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 50 mM CHES, 150 mM sodium chloride, 0.3 mM zinc chloride, 1 mM H2BDH |