5CHI

Crystal structure of PF2046 in complex with ssDNA

Summary for 5CHI

DescriptorUncharacterized protein, DNA (5'-D(P*TP*TP*TP*T)-3'), MAGNESIUM ION, ... (4 entities in total)
Functional Keywordsrnaseh, hydrolase-dna complex, hydrolase/dna
Biological sourcePyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
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Total number of polymer chains6
Total molecular weight80895.1
Authors
Kim, J.S.,Hwang, K.Y.,Lee, W.C. (deposition date: 2015-07-10, release date: 2016-08-10, Last modification date: 2017-09-27)
Primary citation
Kim, J.S.,Sambalkhundev, G.D.,Kim, S.H.,Han, A.,Ko, S.M.,Hwang, K.Y.,Lee, W.C.
Structural basis of two-nucleotide removal of ssDNA by a cryptic RNase H fold 3'-5' exonuclease PF2046 from Pyrococcus furiosus
to be published,
Experimental method
X-RAY DIFFRACTION (2.472 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.232131.2%2.6%1.1%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5chi
no rotation
Molmil generated image of 5chi
rotated about x axis by 90°
Molmil generated image of 5chi
rotated about y axis by 90°