5CEX

Crystal Structure of Fab 32H+109L, a putative precursor of the PGT121 family of potent HIV-1 antibodies

5CEX の概要

• エントリーDOI: 10.2210/pdb5cex/pdb
• 関連するPDBエントリー: 5CEY 5CEZ
• 分子名称: Antibody 9H+3L Fab light chain, Antibody 9H+3L Fab heavy chain, TETRAETHYLENE GLYCOL, ... (7 entities in total)
• 機能のキーワード: hiv-1, immune system, antibody
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 99166.38

構造登録者

Garces, F.,Wilson, I.A. (登録日: 2015-07-07, 公開日: 2015-12-23, 最終更新日: 2024-10-30)

主引用文献

Garces, F.,Lee, J.H.,de Val, N.,Torrents de la Pena, A.,Kong, L.,Puchades, C.,Hua, Y.,Stanfield, R.L.,Burton, D.R.,Moore, J.P.,Sanders, R.W.,Ward, A.B.,Wilson, I.A.
Affinity Maturation of a Potent Family of HIV Antibodies Is Primarily Focused on Accommodating or Avoiding Glycans.
Immunity, 43:1053-1063, 2015

PubMed Abstract: The high-mannose patch on the HIV-1 envelope (Env) glycoprotein is the epicenter for binding of the potent broadly neutralizing PGT121 family of antibodies, but strategies for generating such antibodies by vaccination have not been defined. We generated structures of inferred antibody intermediates by X-ray crystallography and electron microscopy to elucidate the molecular events that occurred during evolution of this family. Binding analyses revealed that affinity maturation was primarily focused on avoiding, accommodating, or binding the N137 glycan. The overall antibody approach angle to Env was defined very early in the maturation process, yet some variation evolved in the PGT121 family branches that led to differences in glycan specificities in their respective epitopes. Furthermore, we determined a crystal structure of the recombinant BG505 SOSIP.664 HIV-1 trimer with a PGT121 family member at 3.0 Å that, in concert with these antibody intermediate structures, provides insights to advance design of HIV vaccine candidates.

PubMed: 26682982
DOI: 10.1016/j.immuni.2015.11.007

実験手法

X-RAY DIFFRACTION (2.105 Å)