5CB7

Crystallographic structure of human rotavirus K8 VP8* in complex with A-type HBGA

Summary for 5CB7

• Entry DOI: 10.2210/pdb5cb7/pdb
• Descriptor: Outer capsid protein VP4, alpha-L-fucopyranose-(1-2)-[2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)]alpha-D-galactopyranose, 2-acetamido-2-deoxy-alpha-D-galactopyranose, ... (8 entities in total)
• Functional Keywords: carbohydrate-recognizing protein, lectin, rotavirus, vp8*, viral protein, sugar binding protein
• Biological source: Rotavirus A (strain Human/Japan/K8/1977 G1-P3A[9]-Ix-Rx-Cx-Mx-A1-Nx-Tx-Ex-H3) (RV-A)
• Total number of polymer chains: 2
• Total formula weight: 38638.26

Authors

Yu, X.,Blanchard, H. (deposition date: 2015-06-30, release date: 2016-06-08, Last modification date: 2024-03-06)

Primary citation

Yu, X.,Mishra, R.,Holloway, G.,von Itzstein, M.,Coulson, B.S.,Blanchard, H.
Substantial Receptor-induced Structural Rearrangement of Rotavirus VP8*: Potential Implications for Cross-Species Infection.
Chembiochem, 16:2176-2181, 2015

PubMed Abstract: Rotavirus-cell binding is the essential first step in rotavirus infection. This binding is a major determinant of rotavirus tropism, as host cell invasion is necessary to initiate infection. Initial rotavirus-cell interactions are mediated by carbohydrate-recognizing domain VP8* of the rotavirus capsid spike protein VP4. Here, we report the first observation of significant structural rearrangement of VP8* from human and animal rotavirus strains upon glycan receptor binding. The structural adaptability of rotavirus VP8* delivers important insights into how human and animal rotaviruses utilize the wider range of cellular glycans identified as VP8* binding partners. Furthermore, our studies on rotaviruses with atypical genetic makeup provide information expected to be critical for understanding the mechanisms of animal rotavirus gene emergence in humans and support implementation of epidemiologic surveillance of animal reservoirs as well as future vaccination schemes.

PubMed: 26250751
DOI: 10.1002/cbic.201500360

Experimental method

X-RAY DIFFRACTION (1.35 Å)