5C97

Insulin regulated aminopeptidase

5C97 の概要

• エントリーDOI: 10.2210/pdb5c97/pdb
• 関連するPDBエントリー: 4P8Q 4PJ6 4Z7I
• 分子名称: Leucyl-cystinyl aminopeptidase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ZINC ION, ... (5 entities in total)
• 機能のキーワード: aminopeptidase, antigen presentation, hydrolase, irap
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 214006.36

構造登録者

Mpakali, A.,Saridakis, E.,Harlos, K.,Zhao, Y.,Stratikos, E. (登録日: 2015-06-26, 公開日: 2015-08-26, 最終更新日: 2024-11-20)

主引用文献

Mpakali, A.,Saridakis, E.,Harlos, K.,Zhao, Y.,Papakyriakou, A.,Kokkala, P.,Georgiadis, D.,Stratikos, E.
Crystal Structure of Insulin-Regulated Aminopeptidase with Bound Substrate Analogue Provides Insight on Antigenic Epitope Precursor Recognition and Processing.
J Immunol., 195:2842-2851, 2015

PubMed Abstract: Aminopeptidases that generate antigenic peptides influence immunodominance and adaptive cytotoxic immune responses. The mechanisms that allow these enzymes to efficiently process a vast number of different long peptide substrates are poorly understood. In this work, we report the structure of insulin-regulated aminopeptidase, an enzyme that prepares antigenic epitopes for cross-presentation in dendritic cells, in complex with an antigenic peptide precursor analog. Insulin-regulated aminopeptidase is found in a semiclosed conformation with an extended internal cavity with limited access to the solvent. The N-terminal moiety of the peptide is located at the active site, positioned optimally for catalysis, whereas the C-terminal moiety of the peptide is stabilized along the extended internal cavity lodged between domains II and IV. Hydrophobic interactions and shape complementarity enhance peptide affinity beyond the catalytic site and support a limited selectivity model for antigenic peptide selection that may underlie the generation of complex immunopeptidomes.

PubMed: 26259583
DOI: 10.4049/jimmunol.1501103

実験手法

X-RAY DIFFRACTION (3.37 Å)