5C97
Insulin regulated aminopeptidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.000, 260.151, 73.360 |
Unit cell angles | 90.00, 111.61, 90.00 |
Refinement procedure
Resolution | 68.204 - 3.370 |
R-factor | 0.2198 |
Rwork | 0.216 |
R-free | 0.28530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p8q |
RMSD bond length | 0.014 |
RMSD bond angle | 2.191 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.270 | 3.460 |
High resolution limit [Å] | 3.370 | 3.370 |
Rmerge | 0.058 | 0.720 |
Number of reflections | 33870 | |
<I/σ(I)> | 16.5 | 2.7 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 6.8 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 10% w/v PEG 4000, 20% v/v glycerol, 0.1M bicine/Trizma base, 0.2 M sodium L-glutamate, 0.2M DL-alanine, 0.2M glycine, 0.2M DL-lysine HCl, 0.2M DL-serine |