5C0W

Structure of a 12-subunit nuclear exosome complex bound to single-stranded RNA substrates

5C0W の概要

• エントリーDOI: 10.2210/pdb5c0w/pdb
• 分子名称: Exosome complex component RRP45, Exosome complex exonuclease DIS3, Exosome complex exonuclease RRP6, ... (15 entities in total)
• 機能のキーワード: hydrolase, rna, nuclease, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 514705.46

構造登録者

Makino, D.L.,Conti, E. (登録日: 2015-06-12, 公開日: 2015-07-29, 最終更新日: 2024-01-10)

主引用文献

Makino, D.L.,Schuch, B.,Stegmann, E.,Baumgartner, M.,Basquin, C.,Conti, E.
RNA degradation paths in a 12-subunit nuclear exosome complex.
Nature, 524:54-58, 2015

PubMed Abstract: The eukaryotic exosome is a conserved RNA-degrading complex that functions in RNA surveillance, turnover and processing. How the same machinery can either completely degrade or precisely trim RNA substrates has long remained unexplained. Here we report the crystal structures of a yeast nuclear exosome containing the 9-subunit core, the 3'-5' RNases Rrp44 and Rrp6, and the obligate Rrp6-binding partner Rrp47 in complex with different RNAs. The combined structural and biochemical data of this 12-subunit complex reveal how a single-stranded RNA can reach the Rrp44 or Rrp6 active sites directly or can bind Rrp6 and be threaded via the central channel towards the distal RNase Rrp44. When a bulky RNA is stalled at the entrance of the channel, Rrp6-Rrp47 swings open. The results suggest how the same molecular machine can coordinate processive degradation and partial trimming in an RNA-dependent manner by a concerted swinging mechanism of the two RNase subunits.

PubMed: 26222026
DOI: 10.1038/nature14865

実験手法

X-RAY DIFFRACTION (4.6 Å)