5BWI

Crystallographic structure of a bacterial heparanase

Summary for 5BWI

• Entry DOI: 10.2210/pdb5bwi/pdb
• Descriptor: Glycoside Hydrolase Family 79, GLYCEROL, ACETATE ION, ... (5 entities in total)
• Functional Keywords: glucuronidase, hydrolase
• Biological source: Burkholderia pseudomallei
• Total number of polymer chains: 2
• Total formula weight: 91089.07

Authors

Yu, X.,Blanchard, H. (deposition date: 2015-06-08, release date: 2015-10-28, Last modification date: 2023-09-27)

Primary citation

Bohlmann, L.,Tredwell, G.D.,Yu, X.,Chang, C.W.,Haselhorst, T.,Winger, M.,Dyason, J.C.,Thomson, R.J.,Tiralongo, J.,Beacham, I.R.,Blanchard, H.,von Itzstein, M.
Functional and structural characterization of a heparanase.
Nat.Chem.Biol., 11:955-957, 2015

PubMed Abstract: We report the structural and functional characterization of a novel heparanase (BpHep) from the invasive pathogenic bacterium Burkholderia pseudomallei (Bp), showing ∼24% sequence identity with human heparanase (hHep). Site-directed mutagenesis studies confirmed the active site resi-dues essential for activity, and we found that BpHep has specificity for heparan sulfate. Finally, we describe the first heparanase X-ray crystal structure, which provides new insight into both substrate recognition and inhibitor design.

PubMed: 26565989
DOI: 10.1038/nchembio.1956

Experimental method

X-RAY DIFFRACTION (1.6 Å)