5BTT
Switching GFP fluorescence using genetically encoded phenyl azide chemistry through two different non-native post-translational modifications routes at the same position.
Summary for 5BTT
Entry DOI | 10.2210/pdb5btt/pdb |
Descriptor | Green fluorescent protein, SULFATE ION, GLYCEROL, ... (5 entities in total) |
Functional Keywords | synthetic biology, photocontrol, optogenetics, unnatural amino acids, protein fluorescence, sfgfp, fluorescent protein |
Biological source | Aequorea victoria (Jellyfish) More |
Total number of polymer chains | 2 |
Total formula weight | 52949.04 |
Authors | Hartley, A.M.,Worthy, H.L.,Reddington, S.C.,Rizkallah, P.J.,Jones, D.D. (deposition date: 2015-06-03, release date: 2016-07-13, Last modification date: 2017-05-10) |
Primary citation | Hartley, A.M.,Worthy, H.L.,Reddington, S.C.,Rizkallah, P.J.,Jones, D.D. Molecular basis for functional switching of GFP by two disparate non-native post-translational modifications of a phenyl azide reaction handle. Chem Sci, 7:6484-6491, 2016 Cited by PubMed: 28451106DOI: 10.1039/c6sc00944a PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.14 Å) |
Structure validation
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