5BOA

Crystal Structure of the Meningitis Pathogen Streptococcus suis adhesion Fhb bound to the disaccharide receptor Gb2

Summary for 5BOA

• Entry DOI: 10.2210/pdb5boa/pdb
• Related: 5BOB
• Descriptor: Translation initiation factor 2 (IF-2 GTPase), alpha-D-galactopyranose-(1-4)-beta-D-galactopyranose (2 entities in total)
• Functional Keywords: protein-polysaccharide complex, sugar binding protein
• Biological source: Streptococcus suis (strain 05ZYH33)
• Total number of polymer chains: 6
• Total formula weight: 155558.90

Authors

Zhang, C.,Yu, Y.,Yang, M.,Jiang, Y. (deposition date: 2015-05-27, release date: 2016-05-04, Last modification date: 2023-11-08)

Primary citation

Zhang, C.,Hao, H.,Yu, Y.,Kong, D.,Chen, S.,Jiang, H.,Yuan, Y.,Zheng, Y.,Yang, M.,Jiang, Y.
Structural basis of the interaction between the meningitis pathogen Streptococcus suis adhesin Fhb and its human receptor.
Febs Lett., 590:1384-1392, 2016

PubMed Abstract: The recently identified Streptococcus suis adhesin factor H-binding protein (Fhb) targets the host cellular receptor glycolipid GbO3 through its N terminus. However, it is unclear how Fhb interacts with its receptor. Here, we determined the complex structure of factor H-binding protein receptor-binding domain (Fhb RBD) with Gb2, an analog of its receptor, revealing that Gb2 binds in a pocket of the β sandwich core domain. We identified the key residues for Fhb RBD receptor binding using mutagenesis and isothermal titration calorimetry. Mutagenesis analyses indicated that Fhb binds to Gb2 mainly through hydrogen and hydrophobic interactions. Our findings provided structural insights into the Fhb-mediated host-pathogen interactions of S. suis.

PubMed: 27086582
DOI: 10.1002/1873-3468.12174

Experimental method

X-RAY DIFFRACTION (2.708 Å)