5BKA

2.1 Angstrom structure of ActVI-ORFA from Streptomyces Coelicolor

5BKA の概要

• エントリーDOI: 10.2210/pdb5bka/pdb
• 分子名称: Hydroxylacyl-CoA dehydrogenase (2 entities in total)
• 機能のキーワード: actinorhodin, benzoisochromaquinone, oxidoreductase
• 由来する生物種: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 97752.10

構造登録者

Vuksanovic, N.,Silvaggi, N.R. (登録日: 2019-06-02, 公開日: 2020-06-03, 最終更新日: 2024-10-30)

主引用文献

Vuksanovic, N.,Zhu, X.,Serrano, D.A.,Siitonen, V.,Metsa-Ketela, M.,Melancon 3rd, C.E.,Silvaggi, N.R.
Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis.
Acta Crystallogr.,Sect.F, 76:372-383, 2020

PubMed Abstract: Proteins belonging to the NTF2-like superfamily are present in the biosynthetic pathways of numerous polyketide natural products, such as anthracyclins and benzoisochromanequinones. Some have been found to be bona fide polyketide cyclases, but many of them have roles that are currently unknown. Here, the X-ray crystal structures of three NTF2-like proteins of unknown function are reported: those of ActVI-ORFA from Streptomyces coelicolor A3(2) and its homologs Caci_6494, a protein from an uncharacterized biosynthetic cluster in Catenulispora acidiphila, and Aln2 from Streptomyces sp. CM020, a protein in the biosynthetic pathway of alnumycin. The presence of a solvent-accessible cavity and the conservation of the His/Asp dyad that is characteristic of many polyketide cyclases suggest a potential enzymatic role for these enzymes in polyketide biosynthesis.

PubMed: 32744249
DOI: 10.1107/S2053230X20009814

実験手法

X-RAY DIFFRACTION (2.11 Å)