5BK2

Crystal structure of maltose binding protein in complex with a peristeric synthetic antibody

Summary for 5BK2

• Entry DOI: 10.2210/pdb5bk2/pdb
• Related PRD ID: PRD_900001
• Descriptor: Maltose-binding periplasmic protein, sAB Heavy Chain, sAB Light Chain, ... (8 entities in total)
• Functional Keywords: maltose binding protein, conformation specific synthetic antibody, fab fragment, sugar binding protein
• Biological source: Escherichia coli; More
• Cellular location: Periplasm: P0AEX9
• Total number of polymer chains: 6
• Total formula weight: 188842.01

Authors

Mukherjee, S.,Kossiakoff, A.A. (deposition date: 2017-09-12, release date: 2018-01-17, Last modification date: 2024-11-06)

Primary citation

Mukherjee, S.,Griffin, D.H.,Horn, J.R.,Rizk, S.S.,Nocula-Lugowska, M.,Malmqvist, M.,Kim, S.S.,Kossiakoff, A.A.
Engineered synthetic antibodies as probes to quantify the energetic contributions of ligand binding to conformational changes in proteins.
J. Biol. Chem., 293:2815-2828, 2018

PubMed Abstract: Conformational changes in proteins due to ligand binding are ubiquitous in biological processes and are integral to many biological systems. However, it is often challenging to link ligand-induced conformational changes to a resulting biological function because it is difficult to distinguish between the energetic components associated with ligand binding and those due to structural rearrangements. Here, we used a unique approach exploiting conformation-specific and regio-specific synthetic antibodies (sABs) to probe the energetic contributions of ligand binding to conformation changes. Using maltose-binding protein (MBP) as a model system, customized phage-display selections were performed to generate sABs that stabilize MBP in different conformational states, modulating ligand-binding affinity in competitive, allosteric, or peristeric manners. We determined that the binding of a closed conformation-specific sAB (sAB-11M) to MBP in the absence of maltose is entropically driven, providing new insight into designing antibody-stabilized protein interactions. Crystal structures of sABs bound to MBP, together with biophysical data, delineate the basis of free energy differences between different conformational states and confirm the use of the sABs as energy probes for dissecting enthalpic and entropic contributions to conformational transitions. Our work provides a foundation for investigating the energetic contributions of distinct conformational dynamics to specific biological outputs. We anticipate that our approach also may be valuable for analyzing the energy landscapes of regulatory proteins controlling biological responses to environmental changes.

PubMed: 29321208
DOI: 10.1074/jbc.RA117.000656

Experimental method

X-RAY DIFFRACTION (2.6 Å)